Independent evaluation of emerging extracellular vesicle technologies.
EV-iTEC collaborates with academic and industry partners to evaluate EV technologies under biologically relevant conditions. Our focus is on orthogonal validation, cross-platform comparison, fraction-resolved workflows and integrated EV quality control.
We are particularly interested in testing new methods, instruments, kits or software that may improve extracellular vesicle isolation, characterization, phenotyping or data interpretation.
Our Evaluation Approach
Biologically Relevant Samples
Validation across diverse EV sample matrices:
- Cell culture supernatants
- Biofluids, including plasma, urine and CSF
- Organoid culture media
- Bacterial EV preparations
Evaluation under realistic sample conditions.
Method Comparison
Orthogonal benchmarking against established EV workflows.
- Head-to-head comparison
- Existing EV methods as reference workflows
- Statistical comparison where applicable
- Isolation and characterization workflow assessment
Critical evaluation of workflow strengths and limitations.
Standardized Documentation
Transparent and reproducible EV workflow documentation.
- Method and parameter documentation
- EV-QC metrics
- Integrated reporting workflows
- Publication-oriented data structure
Documentation aligned with current EV reporting standards.
Current Evaluation Program
ACTIVE: 2026–2027
CytoFLEX nano Evaluation Program
EV-iTEC currently hosts a one-year CytoFLEX nano evaluation workflow to benchmark advanced single-EV flow cytometry approaches in comparison with established EV characterization platforms.
Current evaluation focus:
- Sub-100 nm EV detection using violet side scatter
- Comparison with NanoFCM-based single-particle analysis
- Performance across different EV sample types
- Multi-parameter fluorescence phenotyping
- Integration into EV-iTEC QC and reporting workflows
Evaluation results are used to refine orthogonal EV-QC and cross-platform validation strategies.
OPEN FOR DISCUSSION
Emerging EV Technologies
We are open to discussing evaluation projects involving:
- EV isolation platforms
- Single-particle analysis systems
- Detection or phenotyping reagents
- EV workflow software
- Analytical or reporting tools
Typical duration: 6 -12 months
Collaboration Formats
Possible Project Scope
- Method validation with biological replicates
- Benchmarking against established EV workflows
- Protocol and workflow optimization
- Technical documentation of key parameters
- EV-QC integration and reporting
- Data structure suitable for scientific publications
Typical Collaboration Setup
- Provision of instrument, kit, software or analytical workflow
- Technical introduction and support by the partner
- Budget for consumables where required
- Joint definition of evaluation goals
- Transparent discussion of data use and reporting
Project duration: typically 6–12 months
Scientific Principles
- Orthogonal validation instead of single-method interpretation
- Awareness of isolation-dependent bias and sample matrix effects
- Fraction-resolved analysis where bulk measurements are insufficient
- Integration of particle, protein, fluorescence and workflow-level QC metrics
- Critical interpretation of what a method can and cannot demonstrate
Interested in a Collaboration?
We are open to discussing evaluation projects involving EV technologies, analytical workflows and orthogonal validation strategies.