Internal EV-iTEC tool for descriptive quality assessment of EV preparations.
Not intended for clinical or regulatory QC decisions.
EV Quick QC
Internal EV-iTEC tool for descriptive quality assessment of EV preparations (PPR, Triton integrity, phenotyping readiness & QC index).
How to use this tool
- Enter source, volume, particle concentration and, if available, mode size.
- Add protein amount / concentration and optional Triton integrity data for a full QC index.
- Click Calculate to obtain summary, phenotyping readiness and QC index. Export data via TSV or print/PDF if needed.
This tool is intended for internal EV-iTEC benchmarking and training. It does not replace method-specific controls and is not validated for clinical or regulatory decision-making.
Source tunes how “strict” protein background is interpreted (no hard cut-offs).
Scientific notation allowed (1.2e11, 1,2e11).
Centers: NTA μ≈120 nm (±50); NanoFCM μ≈70 nm (±25); slope k=10.
Use a low Triton concentration (0.1 % v/v). Higher detergent can generate additional
micelles/particles visible in NTA. Consider pre-diluting the Triton-treated sample to reduce
detergent-derived background. Always measure a matching buffer + Triton control and enter it
here; integrity VI = (1 − (sample−ctrl)/pre) × 100 % is included in the QC index.
If both are given, total amount is used. If only conc is given, total = conc × volume.