Your partner for robust, reproducible extracellular vesicle research from experimental design to high-quality data interpretation. We combine deep technical know-how, established EV workflows, and Core Facility infrastructure to support your EV projects at every stage.
Consultation Services
We support you in planning, executing, and interpreting EV-related experiments that meet current MISEV recommendations and international standards.
- Strategic planning of EV experiments
- Matching isolation and characterization methods to your question
- QC and reporting aligned with MISEV
- Support from pilot to publication
Experiment Design & MISEV Compliance
We help optimize study design, choose appropriate EV isolation strategies, define QC checkpoints, and implement reporting structures aligned with current MISEV recommendations.
Post-Experiment Review & Troubleshooting
Interpretation of EV data, identification of technical issues, refinement of workflows, and troubleshooting of unexpected QC patterns or unusual experimental outcomes.
EV Isolation Methods
We provide a flexible portfolio of EV isolation workflows tailored to sample type, volume, and downstream applications – from differential ultracentrifugation to advanced free-flow electrophoresis.
Differential and Density Gradient Ultracentifugation
- High-speed separation using Beckman Coulter Optima XPN-80 and Optima Max-XP ultracentrifuges for EV recovery.
- Pure EV fractions can be isolated using iodixanol (OptiPrep™) or sucrose gradients, including determination of density by refractometry
Tangential Flow Filtration
- Scalable method for concentrating EVs from medium to large volumes. Gentle enrichmentwith defined cut-offs.
Chromatography
- SEC: Gravity-based homemade Sepharose CL-4B columns and Izon columns with an automatic fraction collector are available for precise separation.
- ÄKTA Start system with CaptoCore 700 for reproducible, high-purity EV preparations
Free-flow Electrophoresis
- Powerful, cutting-edge technique used to separate extracellular vesicles based on their surface charge and electrophoretic mobility in a non-invasive manner. This method enables the enrichment of specific EV subpopulations while maintaining their structural integrity and functionality. It is particularly valuable for isolating EVs with subtle biochemical differences, such as variations in charge due to surface proteins or lipid composition.
EV Characterization
- Nanoparticle Tracking Analysis (NTA) Measurement of EV size distribution, concentration and zeta potential using Zetaview. Ideal for particle load, purity comparisons and QC.
- Nano-Flow Cytometry High-sensitivity phenotyping of EV surface markers using fluorophore-conjugated antibodies (e.g., Alexa488, FITC, APC). Many markers available: CD63, CD81, CD29, and more.
- Western Blotting Validation of EV identity and purity via positive and negative markers. Useful for confirming enrichment and ruling out contamination.
- cProtein & Nucleic Acid Quantification Quantification using microBCA and Qubit assays. Ideal for comparing EV cargo and QC across methods.
- Mass Spectrometry Sample Preparation. EV sample cleanup, lysis and QC for proteomics in collaboration with the Institute of Translational Proteomics & Core Facility.
Integrated EV QC & Reporting
We emphasize transparent EV QC and reporting aligned with MISEV standards.
- Support for internal EV-iTEC QC indices
- Recommendations for figure layouts & method sections
- Troubleshooting inconsistent QC patterns