Extracellular vesicle experiments are highly sensitive to cell state, conditioning strategy, isolation workflow, normalization and analytical bias. EV-iTEC therefore provides quality control, experimental design and reporting tools to support transparent, reproducible and method-aware EV workflows, featuring MiTEC (Minimum Information for Transparent EV Characterization).
Before starting an EV experiment, ask yourself:
- What is the biological question?
- What result would contradict my hypothesis?
- Which control would convince a sceptical reviewer?
- Why was this isolation strategy chosen?
- How will the data be normalized?
- Which information must be reported?
EV-iTEC tools support experimental planning, QC and reporting. They do not replace scientific judgement, experimental controls or critical interpretation.
MiTEC was developed by the EV-iTEC Core Facility to support practical experimental planning and structured documentation of extracellular vesicle studies in alignment with MISEV2023 principles. MiTEC is not intended to replace either MISEV or EV-TRACK. While EV-TRACK serves as a community-driven platform for transparent reporting and centralized documentation of completed studies, MiTEC focuses on the earlier stages of experimental design, feasibility assessment, pilot planning, and routine reporting. The two approaches are complementary and together contribute to improved transparency, reproducibility and reporting quality in extracellular vesicle research.
How to use this tool
- Start with the Pre-Experiment Feasibility Check before scheduling EV isolation.
- For new users, new sample sources or large-scale runs, complete the Minimum Pilot Requirement.
- Use the MiTEC Reporting Template to document sample source, collection conditions, isolation workflow and characterization.
MiTEC = Minimum Information for Transparent EV Characterization. This EV-iTEC tool follows the logic of MISEV-oriented transparent reporting, but does not replace expert consultation or project-specific experimental design.
<Important: MiTEC is an EV-iTEC reporting aid designed to support transparent EV documentation in alignment with MISEV principles. It is not a quality certification tool and does not replace MISEV recommendations, experimental controls, biological interpretation or independent scientific judgement.
EV-iTEC sample and collection check
This check supports early feasibility assessment for cell culture, primary biofluids and other EV-containing materials.
Minimum pilot requirement
No scale-up without pilot. For new users, new sample sources, new biofluids or large-scale isolation, a small pilot is required before time-intensive EV workflows.
Recommended pilot items
Pilot score
Pilot notes
MiTEC reporting template
Complete essential fields before submitting data for interpretation. Optional fields improve MISEV-oriented transparency but should not overload routine EV-iTEC documentation.