This EV-iTEC tool supports transparent comparison of extracellular vesicle (EV) isolation workflows using a weighted multi-parameter scoring approach.
The framework is inspired by the comparative analysis presented in:
Preußer et al. Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Isolation Methods. Advanced Healthcare Materials (2026). DOI: 10.1002/adhm.202504684
The tool is intended as an EV-iTEC decision-support and benchmarking aid. It does not establish universal rankings of EV isolation methods and is not a quality certification system.
Results depend on the selected weighting profile, measured parameters and experimental context.
Lipid content (µg/mL or SPV-equivalent): Measured lipid-associated signal of the final EV preparation. Higher values indicate greater lipid recovery.
EV marker enrichment (relative units): Relative enrichment of EV-associated markers determined by proteomics, immunoblotting, ELISA, flow cytometry, NanoFCM or comparable methods. Values should only be compared within the same experimental workflow.
EV Isolation Benchmark Tool
Relative EV-iTEC comparison of EV isolation workflows. Missing values are not scored as zero; available criteria are re-normalized per method.
| Use | Method | Particles/mL | Protein µg/mL | Lipid content µg/mL or SPV-equivalent |
EV marker enrichment relative units |
Workflow effort |
|---|---|---|---|---|---|---|