Equipment & Capabilities

Key instruments at EV-iTEC supporting advanced EV isolation, single-particle characterization, biochemical analysis and method validation.

EV Isolation

Ultracentrifuges

Optima XPN-80 & MAX-XP

Key Specs:

  • Refrigerated high-speed ultracentrifugation
  • Rotors: Type 70 Ti, SW 32 Ti, MLA-80
  • Max g-force: 70,000 – 200,000 × g
  • Supports dUC, density gradients (iodixanol, sucrose)

Applications:

  • Benchmark EV isolation, density-resolved fractionation, method validation.

Chromatography

ÄKTA Start + Gravity Flow

Key Specs:

  • Columns: Sepharose CL-4B, Izon qEV, CaptoCore 700
  • UV detection, automatic fraction collector
  • Flow rate: 0.5–10 mL/min
  • R-based analysis pipeline

Applications:

  • High-purity SEC, matrix comparison, reproducible fractionation.

Tangential Flow Filtration

PALL Minimate

Key Specs:

  • Membranes: 300 kDa & 500 kDa cutoff
  • Max feed volume: up to 2 L
  • Pressure monitoring
  • Gentle EV concentration + buffer exchange

Applications:

  • Pre-enrichment before SEC/UC/FFE, scalable EV concentration.

Free-Flow Electrophoresis

2× FFE Systems

Key Specs:

  • Continuous mode charge-based separation
  • Temperature-controlled chamber
  • Native separation (PBS, HEPES buffers)
  • High capacity (2 units available)

Applications:

  • Subpopulation enrichment, EV heterogeneity, corona studies.

Single-Particle Analysis

🔬 REFERENCE LAB 2026-2027

High-Sensitivity Flow Cytometry

Beckman CytoFlex Nano

Key Specs:

  • Lasers: 405 nm, 488 nm, 561 nm, 638 nm
  • Violet side scatter (VSSC) for sub-100nm detection
  • Multi-parameter fluorescence phenotyping
  • Calibration beads for fluorescence reference

Applications:

  • Method validation vs. nanoFCM, high-sensitivity EV phenotyping, comparative analysis.

Nano Flow Cytometry

NanoFCM NanoAnalyzer

Key Specs:

  • Lasers: 488 nm & 638 nm
  • Scatter sensitivity: down to ~40 nm
  • Fluorescence: FITC/Alexa488, PE, APC/Alexa647
  • Antibody-labeled EV detection
  • Automated data analysis pipeline (R)

Applications:

  • High-throughput EV phenotyping, tetraspanin profiling, surface marker detection.

Nanoparticle Tracking Analysis

ZetaView PMX110

Key Specs:

  • Laser: 488 nm
  • Video-based particle tracking
  • Size detection: 30–1000 nm
  • Zeta potential measurement
  • Triton-integrity testing compatible

Applications:

  • Size distribution, concentration, zeta potential, vesicle integrity QC.

Bead-Based Flow Cytometry

BD FACS Celesta

Key Specs:

  • Lasers: 405 nm, 488 nm, 561 nm
  • Bead-capture assays (MACSPlex, Dynabeads)
  • Multi-color compensation
  • Not for single EV detection

Applications:

  • EV immunophenotyping via bead capture, QC of marker panels.

Biochemical & Metabolic Analysis

Protein Quantification

Micro BCA & Qubit

Key Specs:

  • Micro BCA assay (Thermo Fisher)
  • Qubit fluorometric quantification
  • Western blotting (EV markers)
  • Particle-to-protein ratio (PPR) calculation

Applications:

  • EV purity assessment, QC normalization, Western validation.

Lipid Quantification

SPV Colorimetric Assay

Key Specs:

  • Sulfophosphovanillin reaction
  • 96-well plate format (Byonoy reader)
  • Lipid/protein ratio calculation
  • Lipid/particle normalization

Applications:

  • Lipid-based EV quantification, QC benchmarking, method comparison.

Proteomics Interface

TimsTOF Ultra + NanoElute

Key Specs:

  • EV-QC before proteomics (PPR, integrity)
  • DIA-MS proteomics (collaboration)
  • Sample handover to Translational Proteomics

Applications:

  • EV proteome profiling, biomarker discovery, corona studies, method benchmarking.

Metabolomics Interface

Orbitrap Exploris™

Key Specs:

Applications:

  • EV metabolite profiling, lipid species analysis, metabolic fingerprinting.
Images © respective manufacturers:
– Beckman Coulter (Optima XPN-80, CytoFlex Nano) • Cytiva (ÄKTA Start, Easy TFF System) • FFE Service (FFE) • NanoFCM (NanoAnalyzer) • Particle Metrix (ZetaView PMX110) • BD Biosciences (FACS Celesta)