Internal EV-iTEC tool for descriptive quality assessment of EV preparations
(PPR, Triton integrity, phenotyping readiness and QC index).
The tool supports cautious interpretation of EV preparation quality by integrating
particle-to-protein ratio, detergent-sensitive particle integrity, sizing context
and estimated phenotyping suitability into a descriptive QC framework.
Results are intended to support transparent EV benchmarking, internal comparison
and training workflows while emphasizing methodological limitations and uncertainty
in EV characterization.
Outputs are intended for EV-iTEC internal benchmarking and educational use only.
The tool does not validate EV identity, purity, biological activity or mechanistic interpretation
and is not intended for clinical or regulatory decision-making.
How to use this tool
Enter source, volume, particle concentration and, if available, mode size.
Add protein amount / concentration and, if available, a matched detergent control.
Click Calculate for a descriptive QC overview and material-planning estimate. Export via TSV or print/PDF if needed.
This tool is intended for internal EV-iTEC benchmarking and training. It does not replace
method-specific controls and is not validated for clinical or regulatory decision-making.
Reported as experimental context; no source-specific purity cut-offs are applied.
Scientific notation allowed (1.2e11, 1,2e11).
Reported separately; it is not automatically subtracted from the sample result.
Mode size is shown as measurement context only and is not scored as preparation quality.
â„¹ï¸ NanoFCM and NTA have different detection biases; their modal diameters are not directly interchangeable.
MISEV2023 recommends reporting the detected distribution rather than only mean, median or mode.
Use a validated low detergent concentration and always measure a matching buffer + detergent
control. The calculated event loss describes detergent lability; it does not establish
EV identity or membrane integrity. Detergent can generate micelles or alter particle detection.
If both are given, total amount is used. If only conc is given, total = conc × volume.
Editable EV-iTEC planning value, not a MISEV threshold or universal assay requirement.
Summary
Particles/Protein Ratio:
particles/µg
Material planning
How to interpret this estimate:
Total protein = (given µg) or (conc µg/µL — volume µL).
PPR = total detected particles / total protein. It is descriptive and is not a universal purity metric.
The selected particle target is used only to estimate required sample volume.
Actual assay feasibility also depends on concentration, staining volume, number of controls, marker abundance and instrument sensitivity.
QC overview
Descriptive measurements no composite quality score
The indicators below are intentionally kept separate because particle count, protein,
detergent lability and apparent size have different biases and do not jointly prove EV identity or purity.
Particle-to-protein ratio
Apparent PPR; compare only within matched source, workflow and measurement settings.
Detergent-labile particle fraction
Event loss after matched detergent treatment; not an EV-integrity measurement.
Size measurement
Mode is reported as context; distribution and instrument settings remain essential.
