Internal EV-iTEC tool for descriptive quality assessment of EV preparations
(PPR, Triton integrity, phenotyping readiness and QC index).
The tool supports cautious interpretation of EV preparation quality by integrating
particle-to-protein ratio, detergent-sensitive particle integrity, sizing context
and estimated phenotyping suitability into a descriptive QC framework.
Results are intended to support transparent EV benchmarking, internal comparison
and training workflows while emphasizing methodological limitations and uncertainty
in EV characterization.
Outputs are intended for EV-iTEC internal benchmarking and educational use only.
The tool does not validate EV identity, purity, biological activity or mechanistic interpretation
and is not intended for clinical or regulatory decision-making.
How to use this tool
Enter source, volume, particle concentration and, if available, mode size.
Add protein amount / concentration and optional Triton integrity data for a full QC index.
Click Calculate to obtain summary, phenotyping readiness and QC index. Export data via TSV or print/PDF if needed.
This tool is intended for internal EV-iTEC benchmarking and training. It does not replace
method-specific controls and is not validated for clinical or regulatory decision-making.
Source tunes how "strict" protein background is interpreted (no hard cut-offs).
ℹ️ NanoFCM-Modi ≤ 70 nm sind typisch und nicht per se ein Qualitätsproblem —
NanoFCM detektiert bevorzugt den unteren Bereich der EV-Größenverteilung und
ist nicht direkt mit NTA-Modi vergleichbar.
Use a low Triton concentration (0.1 % v/v). Higher detergent can generate additional
micelles/particles visible in NTA. Consider pre-diluting the Triton-treated sample to reduce
detergent-derived background. Always measure a matching buffer + Triton control and enter it
here; integrity VI = (1 − (sample−ctrl)/pre) × 100 % is included in the QC index.
If both are given, total amount is used. If only conc is given, total = conc × volume.
Summary
Particles/Protein Ratio:
– particles/µg
–
–
Phenotyping readiness (NanoFCM / WB / Proteomics)
–
Scoring (soft, no hard cuts):
Total protein = (given µg) or (conc µg/µL × volume µL).
PPR = total particles / total protein (descriptive; higher ≈ lower relative protein background).
Integrity (Triton) uses VI = (1 − (sample−ctrl)/pre) × 100 %, mapped via sigmoid to 0–1 (midpoint 60 %, k=10).
Size uses a sigmoid around instrument-specific mode sizes (NTA vs NanoFCM).
Protein conc. subscore favours lower apparent protein concentration, source-specific.
QC index = 100 × (0.3·sPPR + 0.4·sVI + 0.2·sSize + 0.1·sConc); only calculated when Triton data are available.
Phenotyping target: ≥ 5×108 total particles; the tool estimates volume needed.
–
Integrated QC index (internal, Triton-based):
– / 100
This index combines Triton sensitivity (integrity), particle/protein loading,
size distribution and protein background. It is intended for internal EV-iTEC
benchmarking and training only; it is not a validated clinical or regulatory metric.
QC index requires Triton integrity data.
Enter post-Triton particle concentration (+ buffer control) to calculate the full
QC index. Without it, only PPR, size and phenotyping readiness are shown.
