EV-iTEC – Extracellular Vesicle Innovation & Technology Core

Independent EV workflow development, technology evaluation and orthogonal characterization for robust extracellular vesicle research.

EV-iTEC focuses on biologically interpretable EV workflows rather than single-method descriptive profiling

EV-iTEC is part of the Institute for Tumor Immunology (ZTI) at Marburg University.

EV Services

Support in EV experiment planning, method selection and MISEV-aligned reporting.

From differential UC to SEC, TFF and FFE – tailored to sample type and downstream application.

NTA, nanoFCM phenotyping, Western blotting and proteomics-ready EV QC workflows.

EV protocols, QC calculators, documentation templates and training material.

Beta testing and validation of emerging EV technologies with instrument manufacturers.

Full EV workflow: UC → DG → TFF → SEC → FFE
Dual single-particle platforms: nanoFCM + CytoFlex Nano (reference lab)
Integrated EV-QC (NTA, nanoFCM, protein loads, integrity)
State-of-the-art instrumentation (2× FFE, ÄKTA Start, CytoFlex Nano, NanoFCM, PMX110)
Method validation & comparative analysis
Experience across research fields (cancer, immunology, biofluids)
R-based analysis pipelines & automated reporting

Extracellular Vesicle Release Within Energetic and Quantitative Constraints: Implications for Function

14/07/2026

A few months ago, I shared a post wondering whether thermodynamics might be a better starting point for understanding #ExtracellularVesicle release than the default assumption…
EV-iTEC Resources & Tools

05/06/2026

🧪 The real story of your EV results begins long before a sample ever reaches the cytometer. Factors such as sample quality, cell state, pre-analytical…
CytoFlexNano now available at EV-iTEC

28/05/2026

Thanks to Beckman Coulter Life Sciences for the opportunity to evaluate their high-sensitivity #FlowCytometer for ExtracellularVesicle analysis. 🔬 The system is now in operation:• Multi-parameter…
Wenn die Methode die Funktion verändert: Warum extrazelluläre Vesikel (EVs) für Medizin und Forschung so wichtig sind

29/01/2026

Nice to see our recent work on extracellular vesicles featured on the website of the Fachbereich Medizin, Philipps-Universität Marburg It is encouraging when method-focused research…
Isolation shapes identity!

21/01/2026

📣 Our new paper is now out in Advanced Healthcare Materials https://advanced.onlinelibrary.wiley.com/doi/10.1002/adhm.202504684 The core message is straightforward and critical for the ExtracellularVesicle field: EV isolation…